The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
We developed the microwell device for measurement of membrane transport of single adherent cells. As the cells in a population (e.g., tumor) is inevitably heterogeneous, a technique to measure the transport activities at a single-cell level is needed. When adherent cells were cultured on the microwells with ∼10 µm diameter, they spread over the opening to form the closed picoliter space. Thus, molecules...
Freshness is an important factor that determines the quality of rice. However, to measure the freshness, the procedures for conventional methods are complicated or the instrument used for the measurement is expensive. If there is a portable device to measure the freshness of rice easily at home, it will be of great help for consumers. In this study, we have developed an electrochemical microdevice...
We present a highly parallel and reproducible method for reconstituting an array of lipid bilayers to analyze membrane transport. We infuse buffer/lipid/buffer solutions sequentially into a microchannel with numerous microchambers in its walls and seal each chamber by a lipid bilayer containing membrane proteins. Due to the small volume of the chamber (2 pL), membrane transport of confined fluorescent...
We describe our effort to realize an assay system to study clathrin-mediated endocytosis (CME) in vitro. Since CME related proteins are known to bind phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), we propose an accessible method to create an array of supported lipid bilayers (SLBs) with different lipid compositions, using a PDMS stamp and a microchannel. Formation of a uniform SLB of a complex...
An integrated microfluidic transport system was constructed based on electrochemical bubble formation. The basic structure of the pumps and valves consisted of three-electrode systems to produce or shrink hydrogen bubbles and a polydimethylsiloxane (PDMS) diaphragm to separate the electrolyte solution from a sample solution in a micro flow channel. The production and extinction of the bubbles caused...
An effective method of shielding CRTs from environmental magnetic fields is presented. The key to this method is the use of L-shaped strips of iron. The L-shaped pieces are arranged around the frame of the CRT. The new shielding method has the effect of reducing and reshaping the field perpendicular to the screen, and reduces color changes. On the other hand, there are no effects from reducing and...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.