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The microscopic analysis of time dependent 3D live cells provides considerable challenges to visualization. Effective visualization can provide insight into the structure and functioning of living cells. The paper presents a case study in which a number of visualization techniques were applied to analyze a specific problem in cell biology: the condensation and de-condensation of chromosomes during...
The surface area of chromosome territories has been suggested as a preferred site for genes, specific RNAs, and accumulations of splicing factors. Here, we investigated the localization of sites of replication within individual chromosome territories.In vivoreplication labeling with thymidine analogues IdUrd and CldUrd was combined with chromosome painting by fluorescentin situhybridization on three-dimensionally...
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