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5-Enolpyruvylshikimate 3-phosphate (EPSP) synthase is an essential enzyme of the shikimate pathway and is the target for the herbicide, glyphosate. Several glyphosate-insensitive forms of Escherichia coli EPSP synthase had been reported in the literatures. In the present study the function and structure of wild type enzyme and three different mutated variants (G96A, A183T and G96A/A183T) were compared...