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The expression of nicotinic acetylcholine receptors (nAChR) in the rat superior cervical ganglion was investigated by Western blotting, immunohistochemistry and non-radioactive in situ hybridization applying probes for the α4-1 and β2 subunit mRNA. Immunoblot analysis of homogenized ganglia using the anti-nAChR antibody WF6 revealed a labeled protein band of apparent molecular weight of 40 kDa which...