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A novel G protein-coupled receptor (BDF3) was isolated from bovine genomic DNA by a combined approach of polymerase chain reaction (PCR) and hybridization techniques. The predicted amino acid sequence is 317 amino acids in length and displays 80% homology to the human α-MSH receptor MC1. Stably transfected into CHO-K1 cells, BDF3 mediates an increase of intracellular cAMP-levels following incubation...
Somatostatin receptor (SSTR) subtype genes are differentially expressed in brain and various peripheral tissues. RNA blotting and semiquantitative PCR analyses have revealed low levels of SSTR1 mRNA in the gastrointestinal tract and relatively high levels in GH 3 anterior pituitary cells. As a first step in the investigation of the regulation of SSTR1 gene expression, we isolated a genomic...
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