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Peroxynitrite, a reactive oxidising species resulting from a reaction between nitric oxide and the superoxide anion, modifies proteins by nitration of certain amino acids such as tyrosine. Tissue factor (TF), a transmembrane protein, is expressed on cells under inflammatory conditions and initiates the coagulation cascade. The extracellular domain of TF is rich in tyrosine. Exposure of recombinant...
We propose that the inhibition of thromboplastin (tissue factor) (TF) by low density lipoprotein (LDL) arises from the lysine-rich, receptor binding domain of apolipoprotein B-100 (apo B-100). This peptide, which we have termed Lysine-Rich Apo B-100 Derived (KRAD-98) peptide corresponds to residues 3121-3217 of apo B-100. cDNA encoding KRAD-98 sequence was cloned into pinpoint Xa(3) and expressed...
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