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Adaptation of continuous cell lines to growth in suspension in a chemically defined medium has significant advantages for design and optimization in manufacturing of biologicals. In this work, changes in the protein expression level during a step-wise adaptation of an adherent Madin Darby canine kidney (MDCK) cell line to suspension growth were analyzed. Therefore, three cell line adaptations were...
A simple and rapid method for the detection of mycoplasmas in mammalian cell cultures has been developed on the basis of the enzymatic activity of adenosine phosphorylase. This enzyme, which has been found in high activity in mycoplasmas in contrast to mammalian cells, catalyzes the transformation of 6-methylpurine deoxyriboside (6-MPDR) into the two cytotoxic products 6-methylpurine and 6-methylpurine...
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