Search results for: M. Sibila

Items from 1 to 20 out of 30 results

article

Retrospective detection of Porcine circovirus 3 (PCV‐3) in pig serum samples from Spain

F. Klaumann, G. Franzo, M. Sohrmann, F. Correa‐Fiz, more

Transboundary and Emerging Diseases > 65 > 5 > 1290 - 1296

Porcine circovirus 3 (PCV‐3) is an emerging circovirus species that has recently been reported in different countries around the world, suggesting a widespread circulation. In this study, sera samples originating from 654 pigs of different production phases and clinical/pathological conditions, submitted for diagnostic purposes between 1996 and 2017, were randomly selected. Detection of PCV‐3 genome...

article

Update on Mycoplasma hyopneumoniae infections in pigs: Knowledge gaps for improved disease control

D. Maes, M. Sibila, P. Kuhnert, J. Segalés, more

Transboundary and Emerging Diseases > 65 > S1 > 110 - 124

Mycoplasma hyopneumoniae (M. hyopneumoniae) is the primary pathogen of enzootic pneumonia, a chronic respiratory disease in pigs. Infections occur worldwide and cause major economic losses to the pig industry. The present paper reviews the current knowledge on M. hyopneumoniae infections, with emphasis on identification and analysis of knowledge gaps for optimizing control of the disease. Close contact...

article

Assessment of Mycoplasma hyopneumoniae-induced Pneumonia using Different Lung Lesion Scoring Systems: a Comparative Review

B. Garcia-Morante, J. Segalés, L. Fraile, A. Pérez de Rozas, more

Journal of Comparative Pathology > 2016 > 154 > 2-3 > 125-134

Mycoplasma hyopneumoniae is the primary aetiological agent of swine enzootic pneumonia (EP) and one of the major contributors to the porcine respiratory disease complex (PRDC). Gross lung lesions in pigs affected by EP consist of cranioventral pulmonary consolidation (CVPC), usually distributed bilaterally in the apical, intermediate, accessory and cranial parts of the diaphragmatic lobes. Several...

chapter

Geometric Reconstruction of the Vascular System of the Rat Brain Imaged by MRA

M.A. Gaudnek, A. Hess, K. Obermayer, L. Budinsky, more

Informatik aktuell > Bildverarbeitung für die Medizin 2005 > Segmentierung > 178-182

Functional Magnetic Resonance Imaging (fMRI) would benefit much from high-quality MR, Angiography (MRA) of the vascular system underlying the functional domain. Ideally fMRI and MRA are performed during the same experiment. Unfortunately, conventional MRA leads to a systematic underestimation of vessels and images with a generally low contrast. Therefore, we developed a method that combines MRA images...

article

Virological and serological characterization of vaccinated and non-vaccinated piglet subpopulations coming from vaccinated and non-vaccinated sows

L. Fraile, J. Segalés, G. Ticó, S. López-Soria, more

Preventive Veterinary Medicine > 2015 > 119 > 3-4 > 153-161

The present study describes the virological and serological profiles of PCV2 vaccinated (V) and non-vaccinated (NV) piglet subpopulations coming from V and NV sows in a PCV2 subclinically infected farm. Four hundred seventy-six piglets born from V or NV sows were further subdivided in a total of four groups: NV sows–NV pigs (NV–NV), NV sows–V pigs (NV–V); V sows–NV pigs (V–NV) and V sows–V pigs (V–V)...

article

Effect of porcine circovirus type 2 (PCV2) load in serum on average daily weight gain during the postweaning period

S. López-Soria, M. Sibila, M. Nofrarías, M. Calsamiglia, more

Veterinary Microbiology > 2014 > 174 > 3-4 > 296-301

Porcine circovirus type 2 (PCV2) is a ubiquitous virus that mainly affects nursery and fattening pigs causing systemic disease (PCV2-SD) or subclinical infection. A characteristic sign in both presentations is reduction of average daily weight gain (ADWG). The present study aimed to assess the relationship between PCV2 load in serum and ADWG from 3 (weaning) to 21 weeks of age (slaughter) (ADWG 3-21)...

article

Comparison of the immunoperoxidase monolayer assay and three commercial ELISAs for detection of antibodies against porcine circovirus type 2

E. Pileri, M. Cortey, F. Rodríguez, M. Sibila, more

The Veterinary Journal > 2014 > 201 > 3 > 429-432

The aim of this study was to compare and correlate antibody titres against porcine circovirus type 2 (PCV2) in porcine sera (n = 1270) obtained by immunoperoxidase monolayer assay (IPMA) with the results of three commercial ELISAs (designated E1, E2 and E3). The correlation between IPMA and ELISA results was excellent (r ² ≥ 0.90). Compared to IPMA, E2 had the highest sensitivity (93.0%),...

article

Humoral response and colostral antibody transfer following ‘one-dose’ pre-mating vaccination of sows against porcine circovirus type-2

M. Sibila, L. Fraile, G. Ticó, S. López-Soria, more

The Veterinary Journal > 2013 > 197 > 3 > 881-883

The objective of this study was to investigate the presence of porcine circovirus type 2 (PCV2) DNA and antibody to the virus in the serum and colostrum of sows vaccinated prior to mating and in their offspring. Seventy-seven sows were randomly distributed into vaccinated (V, n=36) and non-vaccinated (NV, n=41) groups. One week before mating, sows were given a PCV2 vaccine (V group) or PBS (NV group)...

article

Simultaneous Porcine Circovirus Type 2 and Mycoplasma hyopneumoniae Co-inoculation does not Potentiate Disease in Conventional Pigs

M. Sibila, M. Fort, M. Nofrarías, A. Pérez de Rozas, more

Journal of Comparative Pathology > 2012 > 147 > 2-3 > 285-295

The aim of this study was to assess the effect of simultaneous experimental inoculation of porcine circovirus type 2 (PCV2; intranasal delivery) and Mycoplasma hyopneumoniae (Mhyo; transtracheal delivery) into conventional, seropositive 6-week-old piglets. Thirty-six male piglets were assigned randomly to four groups: control (n=6), PCV2 (n=6), Mhyo (n=12) and PCV2+Mhyo (n=12). Blood samples and faecal...

article

Lack of effect of piglet vaccination against Porcine circovirus type 2 (PCV2) on serum viral loads of Torque teno sus virus 2 (TTSuV2)

D. Nieto, M. Aramouni, M. Sibila, L. Fraile, more

Veterinary Microbiology > 2012 > 157 > 1-2 > 8-12

Anelloviruses are small, non-enveloped viruses with circular single stranded DNA, which infect a number of animal species as well as humans. In pigs, two distinct Torque teno sus virus (TTSuV) species have been described so far, being one of them linked to disease occurrence. Specifically, TTSuV2 loads in serum have been found increased in pigs suffering from postweaning multisystemic wasting syndrome...

article

Microarray analysis of mediastinal lymph node of pigs naturally affected by postweaning multisystemic wasting syndrome

L.T. Fernandes, A. Tomás, A. Bensaid, M. Sibila, more

Virus Research > 2012 > 165 > 2 > 134-142

Postweaning multisystemic wasting syndrome (PMWS) is one of the pig diseases with major economic impact worldwide. Clinical, pathologic and some immunologic aspects of this disease are relatively well-known, but the molecular mechanisms underlying the pathogenic mechanisms of the disease are still poorly understood. The objective of the present study was to investigate the global transcriptome changes...

article

Torque teno sus virus 1 and 2 viral loads in postweaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS) affected pigs

M. Aramouni, J. Segalés, M. Sibila, G.E. Martin-Valls, more

Veterinary Microbiology > 2011 > 153 > 3-4 > 377-381

Torque teno viruses (TTV) are small, non-enveloped viruses with a circular single-stranded DNA genome, which are considered non-pathogenic. However, TTVs have been eventually linked to human diseases. TTVs infecting pigs, Torque teno sus virus 1 (TTSuV1) and 2 (TTSuV2), have been recently associated to porcine circovirus diseases (PCVD). To get more insights into such potential disease association,...

article

Genotypic shift of porcine circovirus type 2 from PCV-2a to PCV-2b in Spain from 1985 to 2008

M. Cortey, E. Pileri, M. Sibila, J. Pujols, more

The Veterinary Journal > 2011 > 187 > 3 > 363-368

Changes in porcine circovirus type 2 (PCV-2) genotypes were evaluated before, during and after outbreaks of post-weaning multisystemic wasting syndrome (PMWS) in (1) a retrospective study using pig sera collected in Spain from 1985 to 2008 and (2) a longitudinal study using pig sera collected from two farms in Spain over periods of 7 and 14years. In both studies, there was a rapid genotypic shift...

article

Serological, pathological and polymerase chain reaction studies on Mycoplasma hyopneumoniae infection in the wild boar

M. Sibila, G. Mentaberre, M. Boadella, E. Huerta, more

Veterinary Microbiology > 2010 > 144 > 1-2 > 214-218

There are evidences that wild boar and domestic pig populations share the vulnerability to certain pathogens and, in consequence, the risk of pathogen transmission. Based on serological evidences, it is known that Mycoplasma hyopneumoniae (Mhyo) is able to infect the wild boar. However, if this infection causes lesions and disease in this species is still unknown. The objectives of the present study...

article

Duration of maternally derived antibodies in Toxoplasma gondii naturally infected piglets

I. García-Bocanegra, M. Simon-Grifé, M. Sibila, J.P. Dubey, more

Veterinary Parasitology > 2010 > 170 > 1-2 > 134-136

A longitudinal study was performed to analyze the dynamics of Toxoplasma gondii antibodies in naturally infected piglets from 1 to 25 weeks of age. Seventy-three piglets from 20 seronegative sows (modified agglutination test, MAT <1:25) and 20 naturally infected T. gondii seropositive sows (MAT ≥1:25) were analyzed at 1, 3, 6, 9, 12, 15, 18, 22 and 25 weeks of age. Twenty-six of the 73 piglets...

article

Corrigendum to “A proposal on porcine circovirus type 2 (PCV2) genotype definition and their relation with postweaning multisystemic wasting syndrome (PMWS) occurrence” [Vet. Microbiol. 128 (2008) 23–25]

L. Grau-Roma, E. Crisci, M. Sibila, S. López-Soria, more

Veterinary Microbiology > 2010 > 141 > 1-2 > 194-195

article

A genetically engineered chimeric vaccine against porcine circovirus type 2 (PCV2) improves clinical, pathological and virological outcomes in postweaning multisystemic wasting syndrome affected farms

J. Segalés, A. Urniza, A. Alegre, T. Bru, more

Vaccine > 2009 > 27 > 52 > 7313-7321

The present study describes the effects of a commercially available genetically engineered chimeric vaccine against porcine circovirus type 2 (PCV2) on clinical, pathological and virological features in three multi-site farms suffering from postweaning multisystemic wasting syndrome (PMWS). The vaccine product was able to reduce clinical signs, PCV2 viral load in lymphoid organs and/or sera, and overall...

article

Swine torque teno virus (TTV) infection and excretion dynamics in conventional pig farms

M. Sibila, L. Martínez-Guinó, E. Huerta, A. Llorens, more

Veterinary Microbiology > 2009 > 139 > 3-4 > 213-218

Torque teno virus (TTV) is a non-enveloped, single-stranded DNA (ssDNA) virus infecting human and non-primate species. Two genogroups of TTV (TTV1 and TTV2) have been described in swine so far. In the present study, TTV1 and TTV2 prevalences in serum, and nasal as well as rectal swabs of 55 randomly selected piglets from seven Spanish multi-site farms, were monitored from 1 to 15 weeks of age. Also,...

article

Pig-major acute phase protein and haptoglobin serum concentrations correlate with PCV2 viremia and the clinical course of postweaning multisystemic wasting syndrome

L. Grau-Roma, P.M.H. Heegaard, C.K. Hjulsager, M. Sibila, more

Veterinary Microbiology > 2009 > 138 > 1-2 > 53-61

The aim of the present longitudinal study was to assess the evolution of two acute phase proteins (APPs), pig-major acute phase protein (pig-MAP) and haptoglobin (HPT), in serum from pigs that developed postweaning multisystemic wasting syndrome (PMWS) in comparison to healthy and wasted non-PMWS affected pigs. In addition, evidence of infection with other pathogens and its relation with variations...

article

One dose of a porcine circovirus 2 (PCV2) sub-unit vaccine administered to 3-week-old conventional piglets elicits cell-mediated immunity and significantly reduces PCV2 viremia in an experimental model

M. Fort, M. Sibila, E. Pérez-Martín, M. Nofrarías, more

Vaccine > 2009 > 27 > 30 > 4031-4037

The immunogenicity and efficacy generated by one dose of a PCV2 sub-unit vaccine (Porcilis PCV ^® ) were evaluated in 3-week-old conventional piglets. Vaccination induced both humoral and cell-mediated responses against PCV2, which were increased after the challenge with a PCV2 genotype “b” isolate. High levels of maternally derived antibodies (IPMA≥10log ₂ ) at the time of vaccination...

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Search results for: M. Sibila

Retrospective detection of Porcine circovirus 3 (PCV‐3) in pig serum samples from Spain

Update on Mycoplasma hyopneumoniae infections in pigs: Knowledge gaps for improved disease control

Assessment of Mycoplasma hyopneumoniae-induced Pneumonia using Different Lung Lesion Scoring Systems: a Comparative Review

Geometric Reconstruction of the Vascular System of the Rat Brain Imaged by MRA

Virological and serological characterization of vaccinated and non-vaccinated piglet subpopulations coming from vaccinated and non-vaccinated sows

Effect of porcine circovirus type 2 (PCV2) load in serum on average daily weight gain during the postweaning period

Comparison of the immunoperoxidase monolayer assay and three commercial ELISAs for detection of antibodies against porcine circovirus type 2

Humoral response and colostral antibody transfer following ‘one-dose’ pre-mating vaccination of sows against porcine circovirus type-2

Simultaneous Porcine Circovirus Type 2 and Mycoplasma hyopneumoniae Co-inoculation does not Potentiate Disease in Conventional Pigs

Lack of effect of piglet vaccination against Porcine circovirus type 2 (PCV2) on serum viral loads of Torque teno sus virus 2 (TTSuV2)

Microarray analysis of mediastinal lymph node of pigs naturally affected by postweaning multisystemic wasting syndrome

Torque teno sus virus 1 and 2 viral loads in postweaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS) affected pigs

Genotypic shift of porcine circovirus type 2 from PCV-2a to PCV-2b in Spain from 1985 to 2008

Serological, pathological and polymerase chain reaction studies on Mycoplasma hyopneumoniae infection in the wild boar

Duration of maternally derived antibodies in Toxoplasma gondii naturally infected piglets

Corrigendum to “A proposal on porcine circovirus type 2 (PCV2) genotype definition and their relation with postweaning multisystemic wasting syndrome (PMWS) occurrence” [Vet. Microbiol. 128 (2008) 23–25]

A genetically engineered chimeric vaccine against porcine circovirus type 2 (PCV2) improves clinical, pathological and virological outcomes in postweaning multisystemic wasting syndrome affected farms

Swine torque teno virus (TTV) infection and excretion dynamics in conventional pig farms

Pig-major acute phase protein and haptoglobin serum concentrations correlate with PCV2 viremia and the clinical course of postweaning multisystemic wasting syndrome

One dose of a porcine circovirus 2 (PCV2) sub-unit vaccine administered to 3-week-old conventional piglets elicits cell-mediated immunity and significantly reduces PCV2 viremia in an experimental model

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