Search results for: Tetyana Sulimenko

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Corrigendum to “GIT1/<beta>PIX signaling proteins and PAK1 kinase regulate microtubule nucleation” [Biochim. Biophys. Acta 1863/6PA (2016) 1282–1297]

Markéta Černohorská, Vadym Sulimenko, Zuzana Hájková, Tetyana Sulimenko, more

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research > 2017 > 1864 > 4 > 747

article

GIT1/βPIX signaling proteins and PAK1 kinase regulate microtubule nucleation

Markéta Černohorská, Vadym Sulimenko, Zuzana Hájková, Tetyana Sulimenko, more

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research > 2016 > 1863 > 6(PA) > 1282-1297

Microtubule nucleation from γ-tubulin complexes, located at the centrosome, is an essential step in the formation of the microtubule cytoskeleton. However, the signaling mechanisms that regulate microtubule nucleation in interphase cells are largely unknown. In this study, we report that γ-tubulin is in complexes containing G protein-coupled receptor kinase-interacting protein 1 (GIT1), p21-activated...

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Nuclear γ‐tubulin associates with nucleoli and interacts with tumor suppressor protein C53

Barbora Hořejší, Stanislav Vinopal, Vladimíra Sládková, Eduarda Dráberová, more

Journal of Cellular Physiology > 227 > 1 > 367 - 382

γ‐Tubulin is assumed to be a typical cytosolic protein necessary for nucleation of microtubules from microtubule organizing centers. Using immunolocalization and cell fractionation techniques in combination with siRNAi and expression of FLAG‐tagged constructs, we have obtained evidence that γ‐tubulin is also present in nucleoli of mammalian interphase cells of diverse cellular origins. Immunoelectron...

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Recovery of tubulin functions after freeze-drying in the presence of trehalose

Eduarda Dráberová, Vadym Sulimenko, Tetyana Sulimenko, Konrad J. Böhm, more

Analytical Biochemistry > 2010 > 397 > 1 > 67-72

Microtubules represent cytoplasmic structures that are indispensable for the maintenance of cell morphology and motility generation. Due to their regular structural organization, microtubules have become of great interest for preparation of in vitro nanotransport systems. However, tubulin, the major building protein of microtubules, is a thermolabile protein and is usually stored at −80°C to preserve...

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Tyrosine phosphorylation of plant tubulin

Yaroslav Blume, Alla Yemets, Vadym Sulimenko, Tetyana Sulimenko, more

Planta > 2008 > 229 > 1 > 143-150

Phosphorylation of αβ-tubulins dimers by protein tyrosine kinases plays an important role in the regulation of cellular growth and differentiation in animal cells. In plants, however, the role of tubulin tyrosine phosphorylation is unknown and data on this tubulin modification are limited. In this study, we used an immunochemical approach to demonstrate that tubulin isolated by both immunoprecipitation...

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Expression of beta-tubulin epitope in human sperm with pathological spermiogram

Jana Peknicova, Marie Pexidrova, Alena Kubatova, Pavel Koubek, more

Fertility and Sterility > 2007 > 88 > 4 Supplement > 1120-1128

To determine the location of the corresponding epitope on the tubulin molecule and to find any differences in its exposition in human sperm with normal and pathological spermiograms. The mature spermatozoon exhibits extraordinary structural compartmentalization that is related to the presence of cytoskeletal proteins and has a functional role in connection with fertilization and motility. Previously,...

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Complexes of γ-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells

Vitaliy Kukharskyy, Vadym Sulimenko, Libor Macůrek, Tetyana Sulimenko, more

Experimental Cell Research > 2004 > 298 > 1 > 218-228

Nonreceptor protein tyrosine kinases of the Src family have been shown to play an important role in signal transduction as well as in regulation of microtubule protein interactions. Here we show that γ-tubulin (γ-Tb) in P19 embryonal carcinoma cells undergoing neuronal differentiation is phosphorylated and forms complexes with protein tyrosine kinases of the Src family, Src and Fyn. Elevated expression...

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γ-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms

Lenka Libusová, Tetyana Sulimenko, Vadym Sulimenko, Pavel Hozák, more

Experimental Cell Research > 2004 > 295 > 2 > 375-386

A panel of six anti-peptide antibodies recognizing epitopes in different regions of the γ-tubulin molecule was used for the characterization and localization of γ-tubulin during cell cycle in Leishmania promastigotes. Immunofluorescence microscopy revealed the presence of γ-tubulin in the basal bodies, posterior pole of the cell, and in the flagellum. Furthermore, the antibodies showed punctuate staining...

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Poly(phenylenediamine) Dispersions

Tetyana Sulimenko, Jaroslav Stejskal, Jan Prokeš

Journal of Colloid And Interface Science > 2001 > 236 > 2 > 328-334

The formation and properties of poly(phenylenediamine) colloidal dispersions were investigated. Oxidative dispersion polymerization of 1,3-phenylenediamine dihydrochloride stabilized with poly(N-vinylpyrrolidone) was taken as a reference experiment. Conductivity, temperature, and acidity of the reaction mixture were recorded during the polymerization. Oxidations of all three phenylenediamine bases,...

article

Conductivity of colloidal polyaniline dispersions

Tetyana Sulimenko, Jaroslav Stejskal, Ivo Křivka, Jan Prokeš

European Polymer Journal > 2001 > 37 > 2 > 219-226

Conductivity of polyaniline dispersions stabilized in aqueous medium with poly(N-vinylpyrrolidone) is related to the prediction for the model system composed of conducting spheres dispersed in a continuous medium. The behaviour of as-prepared dispersion, dispersions dialysed against dilute hydrochloric acid and water, and dispersions based on dry particles redispersed in water is reported. An increase...

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