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Artificial cell sheet is utilized as a useful method for tissue engineering. We proposed a novel approach to fabricate the Ca-alginate gel sheet embedded liver cells to mimic the liver lobule tissue. Ca-alginate sheet with hepatic lobule shaped pattern was firstly deposited on a micro-electrode device based on the electrodeposition method. Viability of embedded cells was checked to be maintained more...
In this paper, we propose a new method to construct microchannels inside cell structures. A local melting of cell embedded hydrogel is employed to make microchannels inside cell structures. The fabricated microchannels can be used as vascular-like networks to supply nutrition and oxygen to cells. Electrodes were fabricated using photolithography technique on a glass substrate. The hydrogel to embed...
We propose a new concept “Lab in a Droplet (LiD)” as a micro to nano scale self-assembly technique. The liquid bridge force is used for assembly of microstructures automatically. Micro/nano objects are ejected from an inkjet nozzle and they are automatically assembled inside a droplet by the liquid bridge force. In this paper, we conducted preliminary experiment to check the possibility of LiD. The...
3D assembly of cellular structures is important for the fabrication of biological substitute in tissue engineering. In this paper, a novel rail-guided multi-microrobotic system was proposed for the assembly of cellular structure. The cellular 2-dimensional (2D) module was fabricated by UV illumination of the crosslinkable hydrogel. The coordinated manipulation among the micromanipulators was performed...
Magnetic hydrogel microbeads have wide applications in the fields of biological engineering and regenerative medicine. In the paper, we proposed a new method for fabricating the magnetic hydrogel beads by encapsulating the magnetic resonance imaging (MRI) contrast media inside the polyethylene glycol diacrylate (PEGDA) solution. Then a microfluidic device was prepared for crosslinking the magnetic...
The technique to construct the artificial organ made of cultured cells is required in many fields such as tissue engineering and drug development. However, the tissue-like construction more than 200 µm thick has central hypoxia and results in necrosis because of the limitation of diffusion of materials. Then, we will propose a method to form a tissue-like construction including vascular network in...
We present the fabrication of 3D porous micro-channel for artificial capillary blood vessel model to study the pressure of glomerulus in vitro. The 3D porous micro-channel was fabricated by direct laser writing to mimic the complex structure of capillary blood vessel. It was designed to connect with micro-channels which were fabricated by UV lithography process. The 3D porous micro-channel was made...
We proposed a micro fluidic device to control the position of Caenorhabditis elegans (C. elegans) and to analyze the condition of C. elegans as a bioindicator during its lifespan. In this device, we can filter C. elegans depending on size and control the position of C. elegans to culture and test C. elegans and to measure the capacitance change. The capacitance change depends on the body volume of...
We propose a microfluidic device to analyze the feature of Caenorhabditis elegans (C. elegans) from an electric property. In this device, we assessed the body volume of C. elegans using the microchip which has a capacitance sensor. This device is mainly composed of two parts. One is a tapered micro channel. The tapered micro channel was used to immobilize C. elegans. The other is a pair of electrode...
We propose a microfluidic device to analyze the feature of Caenorhabditis elegans (C. elegans) from an electric property. In this device, we assessed the body size of C. elegans using the microchip in which a capacitance sensor was embedded. This device is mainly composed of two parts. One is a tapered micro channel. The tapered micro channel was used to immobilize C. elegans and directly measure...
Micro/nano robots have been actively studied toward the realization. Now, the micro living organisms have been used as the driving forces for the micro objects. To achieve the fine manipulation of bio driven micro objects, the manipulation methods have to be established. Therefore, the local environmental control technique is desired to manipulate a single cell and to analyze the detailed properties...
We performed in situ penetration of single wild-type yeast cell (W303) using nano-pipette inside an environmental scanning electron microscope (E-SEM). An E-SEM nanorobotic manipulator has been constructed with three units and seven degrees of freedom (DOFs) in total. Micro pulling and FIB etching were used to control the tip size of nano-pipette accurately. Cells were fixed on the cooling stage in...
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