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This work presents a systematic evaluation of the influence of lipids and casein on the performance of a chromatographic capture step for the recovery of a target protein from transgenic milk. Lactoperoxidase (LPO) was spiked at concentrations typical of those to be expected for transgenic proteins in commercial bovine milk and the dynamic adsorption of LPO to fixed beds of SP Sepharose FF studied...
Previous results with the conjugated polymer poly(2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylenevinylene) (MEH-PPV) have shown that this polymer can be efficiently photobleached by UV irradiation in the presence of gaseous reagents such as hydrazine and thiols. These reagents lead to an efficient saturation of CC double bonds, which is utilized for the set-up of color-patterned light emitting devices...
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