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Many applications in biology, medicine, and material science have a demand for a time and spatially resolved detection of luminescence, particularly in the near infrared spectral region. For example, observation of singlet oxygen phosphorescence is important in understanding cell-cell interactions as well as for research involving photo-dynamic therapy. Furthermore, investigating the temporal behavior...
Progress in nanosciences and life sciences is closely related to developments of high resolution imaging techniques. We introduce a technique which produces correlated topography and fluorescence lifetime images with nanometer resolution. Spot sizes below 5 nm are achieved by quenching of the fluorescence with silicon probes of an atomic force microscope which is combined and synchronized with a confocal...
We present diffusion measurements of the fluorescent dye Cy5 in aqueous solutions using conventional fluorescence correlation spectroscopy (FCS) and the recently introduced dual-focus fluorescence correlation spectroscopy (2fFCS). We study the sensitivity of both methods with respect to excitation intensity. Due to the light-driven transitions of Cy5 between fluorescent and non-fluorescent states,...
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