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A new series of gene carriers, polyethylene glycol (PEG)-grafted poly-l-lysine (PLL, mol. wt.=25 000) with three different PEG-grafted ratios (5, 10 and 25 mole%, which means 5, 10 and 25% of ε-amino group of PLL was modified by PEG), was synthesized. These new gene carriers, named comb-shaped PEG-g-PLL copolymer, showed a 5- to 30-fold increase in transfection efficiency compared to PLL alone on...