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The nitrate reductase gene (YNR1) from the yeast H. polymorpha was isolated from a lambda EMBL3 genomic DNA library. As probe a 350 bp DNA fragment synthesized by PCR from H. polymorpha cDNA was used. By DNA sequencing an ORF of 2,577 bp was found. The predicted protein has 859 amino acids and presents high identity with nitrate reductases from other organisms. Chromosomal disruption of YNR1 causes...
Heat shock from 25 o C to 40 o C of Hansenula anomalacells resulted in a rapid and reversible inactivation of the NADPH-nitrate reductase (NR) activity. The inactive enzyme retained partial activity with the non-physiological co-substrates, reduced methyl viologen and reduced flavin mononucleotide. The inactive NR pelleted after centrifugation at 12,000 x g for 30 min and was associated...
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