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The kinetics of transcription initiation in Escherichia coli depend on the duration of two rate-limiting steps, the closed and the open complex formation. In a lac promoter variant, P lac/ara-1 , the kinetics of these steps is controlled by IPTG and arabinose. From in vivo single-RNA measurements, we find that induction affects the mean and normalized variance of the intervals between consecutive...
In vitro studies show that the transcriptional dynamics in Escherichia coli is sensitive to Mg 2+ concentration in the cell. We study in vivo how Mg 2+ affects the production of RNA molecules under the control of the lar promoter, P lar , a lac promoter variant. The target RNA codes for RFP followed by 96 MS2d-GFP binding sites, allowing in vivo detection of individual RNA...
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