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An investigation of the properties of TSH receptors on FRTL 5 cells using affinity labelling with a 125 I-labelled photoactive derivative of TSH is described. Our studies suggest that FRTL 5 cells contain 2 principal types of cell surface TSH receptors. One form, probably a precursor, consists of a single polypeptide chain (M r 120 000) with an intrachain...
Affinity purified, detergent-solubilised porcine TSH receptors have been crosslinked to a 125 I-labelled photoactive derivative of TSH and analysed by gel electrophoresis, gel filtration and sucrose density gradient centrifugation. Our studies suggest that the porcine TSH receptor is made up of a hydrophilic A subunit with an M r about 45000 linked to an amphiphilic B subunit...
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