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For the first time, the relationship between the structural parameters of dye molecule and the enhanced resonance light scattering (RLS) of dye-human serum albumen (HSA) was investigated using chemometrics technique. The data set of the enhanced RLS intensity of the dye-HSA was obtained under the same experimental conditions for 22 dyes. After calculated with HyperChem software and selected by stepwise...
A micellar electrokinetic capillary chromatography (MECC) method for the separation of three active components, imperatorin (IM), isoimperatorin (IO) and scopoletin (SC), in Angelica dahurica Benth and its preparation was developed. The optimum separation for these analytes was achieved using 10 mM borate, 20 mM SDS and 10%(v/v) acetonitrile, with applied voltage of 22 kV. All experiments were performed...
A new methodology based on the coupling of an on-line microwave oven, employed to accelerate low-rate reactions, and artificial neural networks (ANNs), applied to the modeling and optimization of a new flow injection system, is proposed. In comparison with traditional heating, a microwave can accelerate low-rate reactions more remarkably and consume less energy. ANNs with a faster back-propagation...
A simple micellar electrokinetic chromatography (MEKC) method, using a 20 mM borate buffer (pH 8.0) with 25 mM SDS in the presence of 10% (V/V) methanol, was established for the identification and determination of two isomers, alantolactone (AL) and isoalantolactone (IAL), in the extracts of Inula helenium L. and Inula racemosa Hook f. Regression equations revealed linear relationships (correlation...
The dispersion behaviour of solutes (samples and reagents) is influenced by the physical properties and structural parameters of solvents used as carrier streams in a flow-injection system. Chromogenic reagents as indicators, such as 4-(5-chloro-2-pyridylazo)-1,3-diaminobenzene (5-Cl-PADAB), 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (5-Br-PADAP), 2-(2-arsonophenylazo)-7-(2,6-dibromo-4-chlorophenylazo)-1,8-dihydroxynaphtha...
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