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Ice-nucleating bacteria are biological ice nucleators capable of elevating the temperature at which ice crystals form in the body fluids of insects, termed the supercooling point (SCP). In the freeze-intolerant Colorado potato beetle, these bacteria reduce its cold tolerance and, consequently, increase the likelihood of mortality in overwintering adults exposed to subzero temperatures. In this field...
The application of ice-nucleating bacteria as biological control agents for Colorado potato beetles requires the development of media for mass production that optimize bacterial efficacy against overwintering adults. The production method in our laboratory has been limited to a solid medium, nutrient agar supplemented with 2.5% glycerol (NAG), that is known to enhance ice-nucleating activity. In this...
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