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Though the three-dimensional structures of barley α-amylase isozymes AMY1 and AMY2 are very similar, they differ remarkably from each other in their affinity for Ca 2+ and when interacting with substrate analogs. A surface site recognizing maltooligosaccharides, not earlier reported for other α-amylases and probably associated with the different activity of AMY1 and AMY2 toward starch...
The novel C 2 symmetric chiral amine trans-2,6-dimethyl-2,6-diphenylpiperidine has been synthesized via two successive nitrone nucleophilic addition-oxidation sequences, followed by reduction of the intermediate nitroxide. Pure enantiomers have been obtained via resolution with mandelic acid.
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