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A second mammalian gene for the ether-à-go-go (eag) potassium channel has been cloned from the rat, and its predicted protein sequence is 70% identical to that of rat ether-à-go-go1 with a further 10% conservatively replaced residues. The rat eag2 mRNA was predominantly expressed in neural tissue and was not detected in adult skeletal, cardiac, or smooth muscle. Within the brain, its distribution...
Purpose: A novel family of directly-gated ATP-ion channels is emerging. Our goal is to clone, functionally express and characterise new members of this family (P2X purinergic receptors).Methods: Degenerate PCR and low-stringency screening of cDNA libraries was used to isolate new P2X cDNAs. Electrophysiological recordings were made in Xenopus oocytes or transiently transfected HEK293 cells. Tissue...
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