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Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder with population prevalence of approximately 60–70 per 10,000. Data shows that both opioid system function enhancement and opiate administration can result in autistic-like symptoms. Cow milk opioid peptides, including β-casomorphin-7 (BCM7, Tyr-Pro-Phe-Pro-Gly-Pro-Ile), affect the μ-opioid receptor (MOR) and are subjected to degradation...
The component of commercial available master mix most sensitive to unfavorable conditions is a polymerase. Available commercial polymerase chain reaction (PCR) master mixes are generally recommended for storage at −20°C, otherwise they may lose their activity.The aim of the experiment was to verify if storing mixes in adverse and extreme conditions may influence the quality of a PCR product. In the...
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