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The selectins are type I membrane glycoproteins that mediate adhesion of leukocytes and platelets to vascular surfaces (McEver 2001; Vestweber and Blanks 1999). L-selectin is expressed on most leukocytes. E-selectin is expressed on cytokine-activated endothelial cells. P-selectin is rapidly redistributed from membranes of secretory granules to the surfaces of activated platelets and endothelial cells...
We directly compared the attachment and rolling of neutrophils on P-selectin and E-selectin under flow. Neutrophils attached more efficiently and rolled at slower velocities on P-selectin than on E-selectin when the proteins were coated on plastic or expressed on CHO cells. Neutrophils that adhered statically to selectin-expressing CHO cells were detached by increasing shear stress. In contrast,...
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