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Six breeds of swine were used to study the structure of swine leukocyte antigen class I (SLA-I). SLA-I complexes were produced by linking SLA-2 genes and β 2 m genes via a linker encoding a 15 amino acid glycine-rich sequence, (G4S)3, using splicing overlap extension (SOE)-PCR in vitro. The six recombinant SLA-2-linker-β 2 m genes were each inserted into p2X vectors and their expression...
[Gao F.-S., Xu C.-B., Zhang X.-H., Zhang W.-J., Guo D. and Zhang S. 2012 Analysing molecular characteristics of the SLA-1 gene from Chinese Hebao pigs. J. Genet.91, xx–xx]
No information to date is available to elucidate the structure of swine leukocyte antigen class I (SLA-I) molecule which is comprised by a heavy chain of SLA-I non-covalently associated with a light chain, β 2 -microglobulin (β 2 m) proteins. Presently, one of SLA-I gene SLA-2 and β 2 m gene were expressed as soluble maltose binding proteins (MBP-proteins) in a pMAL-p2X/Escherichia...
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