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Steroid ligands are known to affect the interactions of their respective receptors with DNA. In the present study, the possibility of DNA interference in progesterone receptor-ligand interactions was investigated. An oligonucleotide containing a hormone response element (HRE) was shown to decrease the dissociation rate of complexes of [ 3 H]progesterone or [ 3 H]16α,17α-cycloalkanoprogesterones...
Tritium-labeled 16α,17α-cyclohex-3′-en-pregn-4-en-3,20-dione (16α, 17α-cyclohex-3′-eno-progesterone) with specific activity of 44 Ci/mmol, required in studying of binding with progesterone receptors, has been obtained by homogenous catalytic hydrogenation of 16α,17α-cyclohex-3′-eno-pregna-1,4-dien-3,20-dione with gaseous tritium and subsequent separation of the mixture by HPLC. Biological testing...
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