Search results for: Ingrid Fleming

Items from 1 to 20 out of 73 results

article

Inhibition of proline‐rich tyrosine kinase 2 restores cardioprotection by remote ischaemic preconditioning in type 2 diabetes

Ralf Erkens, Dragos Andrei Duse, Amanda Brum, Alexandra Chadt, more

British Journal of Pharmacology > 181 > 21 > 4174 - 4194

Background and Purpose Remote ischaemic preconditioning (rIPC) for cardioprotection is severely impaired in diabetes, and therapeutic options to restore it are lacking. The vascular endothelium plays a key role in rIPC. Given that the activity of endothelial nitric oxide synthase (eNOS) is inhibited by proline‐rich tyrosine kinase 2 (Pyk2), we hypothesized that pharmacological Pyk2 inhibition could...

article

Anomalous K_v7 channel activity in human malignant hyperthermia syndrome unmasks a key role for H₂S and persulfidation in skeletal muscle

Valentina Vellecco, Alma Martelli, Iris Sofia Bibli, Marianna Vallifuoco, more

British Journal of Pharmacology > 177 > 4 > 810 - 823

Background and Purpose Human malignant hyperthermia (MH) syndrome is induced by volatile anaesthetics and involves increased levels of cystathionine β‐synthase (CBS)‐derived H₂S within skeletal muscle. This increase contributes to skeletal muscle hypercontractility. K_v7 channels, expressed in skeletal muscle, may be a molecular target for H₂S. Here, we have investigated the role of K_v7 channels in...

article

AKAP12 deficiency impairs VEGF‐induced endothelial cell migration and sprouting

Peter M. Benz, Yindi Ding, Heike Stingl, Annemarieke E. Loot, more

Acta Physiologica > 228 > 1 > n/a - n/a

Aim Protein kinase (PK) A anchoring protein (AKAP) 12 is a scaffolding protein that anchors PKA to compartmentalize cyclic AMP signalling. This study assessed the consequences of the downregulation or deletion of AKAP12 on endothelial cell migration and angiogenesis. Methods The consequences of siRNA‐mediated downregulation AKAP12 were studied in primary cultures of human endothelial cells as well...

article

The histone demethylase Jarid1b mediates angiotensin II‐induced endothelial dysfunction by controlling the 3′UTR of soluble epoxide hydrolase

Andrea E. Vasconez, Patrick Janetzko, James A. Oo, Beatrice Pflüger‐Müller, more

Acta Physiologica > 225 > 1 > n/a - n/a

Aim The histone demethylase Jarid1b limits gene expression by removing the active methyl mark from histone3 lysine4 at gene promoter regions. A vascular function of Jarid1b is unknown, but a vasoprotective function to inflammatory and hypertrophic stimuli, like angiotensin II (AngII) could be inferred. This hypothesis was tested using Jarid1b knockout mice and the inhibitor PBIT. Methods Mice or...

article

Angiogenesis and vascular stability in eicosanoids and cancer

Jiong Hu, Timo Frömel, Ingrid Fleming

Cancer and Metastasis Reviews > 2018 > 37 > 2-3 > 425-438

Angiogenesis and inflammation are hallmarks of cancer. Arachidonic acid and other polyunsaturated fatty acids (PUFAs) are primarily metabolized by three distinct enzymatic systems initiated by cyclooxygenases, lipoxygenases, and cytochrome P450 enzymes (CYP) to generate bioactive eicosanoids, including prostanoids, leukotrienes, hydroxyeicosatetraenoic acids, and epoxyeicosatrienoic acids. As some...

article

Role of the angiotensin-converting enzyme in the G-CSF-induced mobilization of progenitor cells

Karin Kohlstedt, Caroline Trouvain, Timo Frömel, Thomas Mudersbach, more

Basic Research in Cardiology > 2018 > 113 > 3 > 1-10

In addition to being a peptidase, the angiotensin-converting enzyme (ACE) can be phosphorylated and involved in signal transduction. We evaluated the role of ACE in granulocyte-colony-stimulating factor (G-CSF)-induced hematopoietic progenitor cell (HPC) mobilization and detected a significant increase in mice-lacking ACE. Transplantation experiments revealed that the loss of ACE in the HPC microenvironment...

article

Phosphorylation of vasodilator-stimulated phosphoprotein contributes to myocardial ischemic preconditioning

David Köhler, Sofia-Iris Bibli, Lothar P. Klammer, Judith M. Roth, more

Basic Research in Cardiology > 2018 > 113 > 2 > 1-15

Ischemic preconditioning (IP) is a well-known strategy to protect organs against cell death following ischemia. The previous work has shown that vasodilator-stimulated phosphoprotein (VASP) is involved in cytoskeletal reorganization and that it holds significant importance for the extent of myocardial ischemia reperfusion injury. Yet, the role of VASP during myocardial IP is, to date, not known. We...

article

Redox Control of Renal Metabolism and Transport Function by the NADPH Oxidase Nox4

Flávia Rezende, Oliver Löwe, Niklas Müller, Pedro Felipe Malacarne, more

Free Radical Biology and Medicine > 2017 > 112 > S1 > 174

The NADPH oxidase isoform Nox4 produces H₂O₂, a potent modulator of cellular metabolism. It oxidizes iron and cysteines and is thus able to directly influence the activity of metabolic enzymes or the dynamics of metabolites. Nox4 is highly expressed in the renal cortex and shows a protective role in chronic renal disease whereas its expression is lost in renal pathology. However, its physiological...

article

Role of Müller cell cytochrome P450 2c44 in murine retinal angiogenesis

Jiong Hu, Alexandra Geyer, Sarah Dziumbla, Khader Awwad, more

Prostaglandins & Other Lipid Mediators > 2017 > 133 > C > 93-102

Polyunsaturated fatty acids (PUFA) and their cytochrome P450 (CYP450) metabolites have been linked to angiogenesis and vessel homeostasis. However, the role of individual CYP isoforms and their endogenous metabolites in those processes are not clear. Here, we focused on the role of Cyp2c44 in postnatal retinal angiogenesis and report that Cyp2c44 is highly expressed in Müller glial cells in the retina...

article

The role of eNOS on the compensatory regulation of vascular tonus by H2S in mouse carotid arteries

Elif Ertuna, Annemarieke E. Loot, Ingrid Fleming, Gunay Yetik-Anacak

Nitric Oxide > 2017 > 69 > C > 45-50

The gasotransmitter nitric oxide (NO) has an important role in vascular function and a decrease in its bioavailability is accepted as a main pathological mechanism for cardiovascular diseases. However, other gasotransmitters such as hydrogen sulfide (H₂S) are also generated by the endothelium and can also affect vascular tone and a crosstalk may exist between H₂S and NO. We therefore investigated...

article

Cytochrome P450 enzymes but not NADPH oxidases are the source of the NADPH-dependent lucigenin chemiluminescence in membrane assays

Flávia Rezende, Kim-Kristin Prior, Oliver Löwe, Ilka Wittig, more

Free Radical Biology and Medicine > 2017 > 102 > C > 57-66

Measuring NADPH oxidase (Nox)-derived reactive oxygen species (ROS) in living tissues and cells is a constant challenge. All probes available display limitations regarding sensitivity, specificity or demand highly specialized detection techniques. In search for a presumably easy, versatile, sensitive and specific technique, numerous studies have used NADPH-stimulated assays in membrane fractions which...

article

Calpain 1 cleaves and inactivates prostacyclin synthase in mesenteric arteries from diabetic mice

Voahanginirina Randriamboavonjy, Anastasia Kyselova, Amro Elgheznawy, Sven Zukunft, more

Basic Research in Cardiology > 2017 > 112 > 1 > 1-13

Diabetes is associated with a number of co-morbidities including an increased risk of developing cardiovascular diseases. The activation of Ca²⁺-activated proteases of the calpain family has been implicated in platelet activation associated with diabetes and this study aimed to determine the role of calpain activation in the development of endothelial dysfunction. Diabetes induction in mice attenuated...

article

Cytochrome P450 is the Source of NADPH-Dependent Signal in Cell-Free Assays for Nox Activity

Flavia Rezende, Kim-Kristin Prior, Ilka Wittig, Valentina Strecker, more

Free Radical Biology and Medicine > 2016 > 100 > S > S28-S29

Measuring Nox-derived reactive oxygen species is a constant challenge in redox biology. All probes available display limitations regarding sensitivity, specificity or demand highly specialized technique. To overcome such limitations, numerous studies have used NADPH-stimulated assays in membrane fractions to address Nox activity. In our previous work we found an unchanged activity in a triple Nox...

article

The factor in EDHF: Cytochrome P450 derived lipid mediators and vascular signaling

Ingrid Fleming

Vascular Pharmacology > 2016 > 86 > C > 31-40

Cytochrome P450 (CYP) epoxygenases metabolize arachidonic acid to generate epoxyeicosatrienoic acids (EETs). The latter are biologically active and reported to act as an endothelium-derived hyperpolarizing factor (EDHF) as well as to affect angiogenic and inflammatory signaling pathways. In addition to arachidonic acid, the CYP epoxygenases also metabolize the Ω-3 polyunsaturated fatty acids (PUFAs),...

article

From basic mechanisms to clinical applications in heart protection, new players in cardiovascular diseases and cardiac theranostics: meeting report from the third international symposium on “New frontiers in cardiovascular research”

Hector A. Cabrera-Fuentes, Julian Aragones, Jürgen Bernhagen, Andreas Boening, more

Basic Research in Cardiology > 2016 > 111 > 6 > 1-13

In this meeting report, particularly addressing the topic of protection of the cardiovascular system from ischemia/reperfusion injury, highlights are presented that relate to conditioning strategies of the heart with respect to molecular mechanisms and outcome in patients’ cohorts, the influence of co-morbidities and medications, as well as the contribution of innate immune reactions in cardioprotection...

article

Can erythrocytes release biologically active NO?

Peter M. Benz, Ingrid Fleming

Cell Communication and Signaling > 2016 > 14 > 1 > 1-4

Under physiological conditions, endothelial cells and the endothelial nitric oxide (NO) synthase (eNOS) are the main source of NO in the cardiovascular system. However, several other cell types have also been implicated in the NO-dependent regulation of cell function, including erythrocytes. NO derived from red blood cells has been proposed to regulate erythrocyte membrane fluidity, inhibit platelet...

article

The soluble epoxide hydrolase determines cholesterol homeostasis by regulating AMPK and SREBP activity

Nicole Mangels, Khader Awwad, Annika Wettenmann, Laila Romagueira Bichara Dos Santos, more

Prostaglandins & Other Lipid Mediators > 2016 > 125 > C > 30-39

Inhibition or deletion of the soluble epoxide hydrolase (sEH) has been linked to reduced cholesterol and protection against atherosclerosis. This study set out to identify sEH substrate(s) or product(s), altered in livers from sEH^−/− mice that contribute to these beneficial effects.In livers and isolated hepatocytes, deletion of sEH decreased expression of HMG CoA reductase, fatty acid synthase and...

article

The eNOS signalosome and its link to endothelial dysfunction

Mauro Siragusa, Ingrid Fleming

Pflügers Archiv - European Journal of Physiology > 2016 > 468 > 7 > 1125-1137

Endothelial nitric oxide synthase (eNOS) plays an essential role in the regulation of endothelial function and acts as a master regulator of vascular tone and homeostasis through the generation of the gasotransmitter nitric oxide (NO). The complex network of events mediating efficient NO synthesis is regulated by post-translational modifications and protein-protein interactions. Dysregulation of these...

article

Meeting report from the 2nd International Symposium on New Frontiers in Cardiovascular Research. Protecting the cardiovascular system from ischemia: between bench and bedside

Hector A. Cabrera-Fuentes, Corina Alba-Alba, Julian Aragones, Jürgen Bernhagen, more

Basic Research in Cardiology > 2016 > 111 > 1 > 1-13

Recent advances in basic cardiovascular research as well as their translation into the clinical situation were the focus at the last “New Frontiers in Cardiovascular Research meeting”. Major topics included the characterization of new targets and procedures in cardioprotection, deciphering new players and inflammatory mechanisms in ischemic heart disease as well as uncovering microRNAs and other biomarkers...

article

HIF‐2α attenuates lymphangiogenesis by up‐regulating IGFBP1 in hepatocellular carcinoma

Theresa Geis, Rüdiger Popp, Jiong Hu, Ingrid Fleming, more

Biology of the Cell > 107 > 6 > 175 - 188

Background InformationTumour‐associated lymphangiogenesis was identified as an important clinical determinant for the prognosis of hepatocellular carcinoma (HCC) and significantly influences patient survival. However, in this context, little is known about regulation of lymphangiogenesis by hypoxia‐inducible factors (HIF). In HCC, mainly HIF‐1α was positively correlated with lymphatic invasion and...

Publication date

Last 3 months (1)
Last year (1)
Last 3 years (1)

Close

Date range setting

Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.

From:

To:
Set your own date range

INFONA - science communication portal

Search results for: Ingrid Fleming

Inhibition of proline‐rich tyrosine kinase 2 restores cardioprotection by remote ischaemic preconditioning in type 2 diabetes

Anomalous K_v7 channel activity in human malignant hyperthermia syndrome unmasks a key role for H₂S and persulfidation in skeletal muscle

AKAP12 deficiency impairs VEGF‐induced endothelial cell migration and sprouting

The histone demethylase Jarid1b mediates angiotensin II‐induced endothelial dysfunction by controlling the 3′UTR of soluble epoxide hydrolase

Angiogenesis and vascular stability in eicosanoids and cancer

Role of the angiotensin-converting enzyme in the G-CSF-induced mobilization of progenitor cells

Phosphorylation of vasodilator-stimulated phosphoprotein contributes to myocardial ischemic preconditioning

Redox Control of Renal Metabolism and Transport Function by the NADPH Oxidase Nox4

Role of Müller cell cytochrome P450 2c44 in murine retinal angiogenesis

The role of eNOS on the compensatory regulation of vascular tonus by H2S in mouse carotid arteries

Cytochrome P450 enzymes but not NADPH oxidases are the source of the NADPH-dependent lucigenin chemiluminescence in membrane assays

Calpain 1 cleaves and inactivates prostacyclin synthase in mesenteric arteries from diabetic mice

Cytochrome P450 is the Source of NADPH-Dependent Signal in Cell-Free Assays for Nox Activity

The factor in EDHF: Cytochrome P450 derived lipid mediators and vascular signaling

From basic mechanisms to clinical applications in heart protection, new players in cardiovascular diseases and cardiac theranostics: meeting report from the third international symposium on “New frontiers in cardiovascular research”

Can erythrocytes release biologically active NO?

The soluble epoxide hydrolase determines cholesterol homeostasis by regulating AMPK and SREBP activity

The eNOS signalosome and its link to endothelial dysfunction

Meeting report from the 2nd International Symposium on New Frontiers in Cardiovascular Research. Protecting the cardiovascular system from ischemia: between bench and bedside

HIF‐2α attenuates lymphangiogenesis by up‐regulating IGFBP1 in hepatocellular carcinoma

Filter options

Publication date

Keywords

Data set

Journal

INFONA - science communication portal

Search results for: Ingrid Fleming

Add recipient

Sending message cancelled

Are you sure you want to cancel sending this message?

Send message

Filter options

Publication date

Date range setting

Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.

Keywords

Data set

Journal

Reporting an error / abuse

Sending the report failed

Accessibility options