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Single-chain Fv antibody (scFv) having 2 types of polypeptide linkers with or without rare codons, namely scFv (G 4 S) 3R and scFv No.10 (with rare codons) and scFv (G 4 S) 3 and scFv No.10 NR (without rare codon), were expressed under controllable conditions in batch and fed-batch fermentation, in order to compare volumetric productivity and specific productivity...