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Type 1 fimbriae are one of the most important factors of Escherichia coli adaptation to different niches in the host. Our study indicated that the genetic marker - fimH gene occurred commonly in commensal E. coli derived from healthy humans but expression of the type 1 fimbriae was not observed. Identification of fim structural subunit genes (fimA-fimH) and recombinase fimE and fimB genes showed...
The objective of the study was to examine the characteristics of the resistance profiles of Escherichia coli isolated from healthy pigs from three farms in Western Poland. The sensitivity to 13 antimicrobial agents was tested by a disk diffusion method, and the presence of 13 resistance genes was determined by PCR. The majority of the isolates were multi-resistant. The most common multi-resistance...
Commensal, intestinal E.coli microflora plays a role in maintenance of intestinal balance of the host, is responsible for defending against pathogenic E.coli. This study encompasses the analysis of BOX-PCR fingerprinting patterns, phylogenetic grouping and virulence genes prevalence among commensal E.coli isolates derived from healthy pigs. Altogether, 274 unique E.coli isolates were identified, 110...
The aim of the study was to determine the influence of the presence or the absence of antibiotic input on the emergence and maintenance of resistance in commensal bacteria from food producing animals. The research material constituted E.coli isolates from two animal species: swine at different age from one conventional pig farm with antibiotic input in young pigs and from beef and dairy cattle originated...
The paper describes the prevalence of resistant strains within the genetic structure of E. coli (phylogenetic group A, B1, B2 and D). A total of 200 commensal E. coli strains have been derived from 10 species of healthy animals residing on ZOO Safari Park area, in Świerkocin, Poland. The phylogenetic structure of E. coli has been analysed with the use of a PCR-based method. The strains were tested...
The Rep-PCR fingerprinting method has been applied to identify genomic diversity of 252 E. coli strains derived in the area of a flowing-water basin. The received results show that applying UPGMA and Nearest Neighbour-Joining clustering methods to statistical analysis of rep-PCR fingerprints has made it possible to discriminate and group the strains, revealing a characteristic structure of E. coli...
The rep-PCR fingerprinting method, with the support of ERIC and REP primers, was used to analyse the genomic diversity of 93 E. coli strains isolated from lake water samples drawn at two different depths. The applied UPGMA for DNA analysis did not reveale any genomic similarities between the 48 E. coli strains derived from the subsurface-zone water and the 43 of the bottom-zone water. The considerable...
Introduction: Several lymphoblastoid cell lines with various defects of HLA class II expression had been described, shedding light on regulation of class II gene expression. Here, we present a new lymphoblastoid cell line (LCL) with such a defect, derived from a class II-positive patient.Material and Methods: Lymphoblastoid cell line (LCL) HAJ was derived from peripheral blood lymphocytes of female...
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