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Tris(hydroxymethyl)phosphine [P(CH2OH)3, THP] is demonstrated to be an alternative fixative to glutaraldehyde for preparation of proteinaceous specimens for SEM analysis. THP is easily prepared from commercially-available [P(CH2OH)4]Cl, and unlike many other alkylphosphines, is reasonably air-stable [>7days at pH 7–8]. Experiments carried out with chicken breast muscle, cross-sectioned perpendicular...
N-methyl naphthalimide can be readily cyclomanganated at the 2-position, directed by the adjacent amide O atom. Di-cyclomanganation also occurs readily to attach Mn(CO) 4 groups at both 2, 7 positions. An X-ray structure determination of the mono-substituted example confirmed the five-membered metallocyclic ring. Cleavage of the Mn–C bond by HgCl 2 or ICl generates 2-substituted HgCl...
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