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Background GSK has modified the licensed monovalent bulk manufacturing process for its split-virion inactivated quadrivalent influenza vaccine (IIV4) to harmonize the process among different strains, resulting in an increased number of finished vaccine doses, while compensating for the change from inactivated trivalent influenza vaccine (IIV3) to IIV4. To confirm the manufacturing changes do not alter...
Cell culture is now available as a method for the production of influenza vaccines in addition to eggs. In accordance with currently accepted practice, viruses recommended as candidates for vaccine manufacture are isolated and propagated exclusively in hens' eggs prior to distribution to manufacturers. Candidate vaccine viruses isolated in cell culture are not available to support vaccine manufacturing...
Die Zusammensetzung des aus drei Virusstämmen hergestellten Grippeimpfstoffs ändert sich jedes Jahr und wird zentral von der Weltgesundheitsorganisation (WHO) festgelegt. Die WHO veröffentlicht im Februar die Empfehlung für die Zusammensetzung des Impfstoffs für die nördliche Hemisphäre. Von einem der WHO‐Referenzlabore werden die Referenzstämme für die Impfstoffherstellung bereitgestellt. Influenzaviren...
The RNA polymerase I system forin vivosynthesis of recombinant influenza vRNA molecules was used for the expression of a chimeric protein, consisting of the 341-amino-acid ectodomain of the glycoprotein E2 of classical swine fever virus and the 37-amino-acid C-terminal membrane anchor of the influenza virus hemagglutinin (HA). During infection with an influenza A helper virus the amplified pseudo-viral...
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