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New vaccines are needed to prevent cholera caused by Vibrio cholerae 0139. Attenuated V cholerae 0139 vaccines were made by deleting multiple copies of the cholera-toxin genetic element from two virulent strains of the organism, M010 and A14456. The deletion mutants were further modified by insertion of a construct that encoded the B subunit of cholera toxin, thus generating strains Bengal-3 and VRI-16...
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