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Proliferating cell nuclear antigen (PCNA), a protein intimately involved in both replication and repair, has been identified in eukaryotes at all levels of evolution. Is primary sequence, Drosophila melanogaster PCNA is 73% identical to mammalian PCNA. Moreover, it is able to substitute for mammalian PCNA in at least one intricate cell-free replication assay. Mutations in the gene for Drosophila PCNA,...
Whatman 3MM paper was chemically modified to generate nickel-charged iminodiacetic acid paper (Ni2+-IDA paper). Bacteria were transformed withEscherichia coliexpression plasmids coding for either unmodified proliferating cell nuclear antigen (PCNA) or PCNA containing a genetically engineered polyhistidine tract (his-tag) located at its NH2terminus. They were then grown, induced, and lysed, and macromolecules...
Two 2 -deoxynucleoside 5 -α-methylenephosphonyl-β,γ-diphosphates were synthesized. They were incorporated into the DNA chain by DNA polymerase α from human placenta. Meanwhile, they were not recognized by DNA polymerases ε and β of the same origin as well as by reverse transcriptases from human immunodeficiency virus and avian myeloblastosis virus.
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