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N-methyl-d-aspartate (NMDA) receptors (NMDARs) are highly expressed in the CNS and mediate the slow component of excitatory transmission. The present study was aimed at characterizing the temperature dependence of the kinetic properties of native NMDARs, with special emphasis on the deactivation of synaptic NMDARs. We used patch-clamp recordings to study synaptic NMDARs at layer II/III pyramidal neurons...
N-methyl-d-aspartate (NMDA) receptors are highly expressed in the CNS, mediate the slow component of excitatory transmission and play key roles in synaptic plasticity and excitotoxicity. These ligand-gated ion channels are heteromultimers composed of NR1 and NR2 subunits activated by glycine and glutamate. In this study, patch-clamp recordings were used to study the temperature sensitivity of recombinant...
The effects of high temperature (53-61 o C) on membrane currents (I heat ) or depolarization (V heat ) induced by noxious heat were studied in cultured dorsal root ganglia neurons from neonatal rats using the whole cell patch clamp technique. I heat or V heat produced by 3 s ramps of increasing temperature...
We describe our project the goal of which is development of a method for measurement of temperature in a cellular microvolume and its hardware implementation. The main prerequisite of the designed method is the noninterference with functions of the measured living tissue, The microsensor may contribute to an experimental study of thermodynamically open systems in biology. The proposed method is based...
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