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The mouse alkyladenine DNA glycosylase (Aag) initiates base excision repair with a broad substrate range that includes the highly mutagenic exocyclic etheno DNA base adduct 1,N 6 -ethenodeoxyadenosine (εdA). Previous attempts to determine the in vivo role of Aag in εdA repair were complicated by technological difficulties in measuring low levels of εdA in genomic DNA. Here we describe the...
Butadiene (BD) is an important industrial chemical classified as a probable human carcinogen. Marked species differences in susceptibility to the carcinogenic effects of BD have been observed, possibly due to the differences in its metabolism. In this work, guanine and adenine adducts formed by the reactive metabolites of BD in vitro were isolated and structurally characterized by UV spectroscopy,...
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