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Neutrophils isolated from human peripheral blood added to a monolayer of human endothelial cells (ECV-304 cell line) stimulated with LPS (100 ng ml−1) resulted in: (a) neutrophil activation, measured by spreading and release of leukotriene B4 (LTB4); (b) neutrophil degranulation, measured by release of matrix pro-metalloproteinase-9 (proMMP-9) and (c) loss of the monolayer integrity due to detachment...
Intraplantar injection of 5 or 10 μg of BjV caused local edema in rats that was not affected by ablation of adrenal glands. In addition, no changes in plasma corticosterone levels were observed. Simultaneous injections of the venom into both hindpaws of normal animals, or injections made at varying intervals, resulted in local inflammatory reactions of comparable time-course development and analogous...
The effect of Bothrops jararaca crude venom (BjV) on the cellular component of inflammatory responses was investigated in vivo and in vitro. In vivo leukocyte accumulation and release of eicosanoids (thromboxane A 2 , TXA 2 , and leukotriene B 4 , LTB 4 ) at the site of injection of the venom were assessed using the air pouch method in rats. Administration of BjV...
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