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To assess whether extracellular microRNAs (miRNAs) can be accurately profiled from spent blastocyst culture media (SBM) and used as embryonic biomarkers.Prospective cohort study.Private and academic in vitro fertilization centers.Inner cell mass-free trophectoderm (TE) samples and their relative SBM from five good-quality human blastocysts.Protocol for miRNA purification and analysis based on quantitative...
Discordant growth is a common complication of monochorionic/diamniotic pregnancies; in approximately 50% of cases, the cause is unknown. The case presented here suggests that discordant growth of monozygotic twins could start during preimplantation development. Two inner cell masses (ICMs) within the same blastocyst may originate in uneven splitting of a single “parental” ICM, or the two ICMs may...
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