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We hypothesized that targeted mutation of the endothelial nitric oxide synthase (eNOS) gene would reduce Akt-related signaling events in skeletal muscle cells, compared to wild type (WT) controls. Results show that slow myosin heavy chain (type I/β) expression and the abundance of slow-twitch fibers are reduced in plantaris muscle of eNOS −/− mice, compared to WT. Further, basal phosphorylation...
Skeletal muscle regenerative potential is reduced with aging. We hypothesized that in vitro activation of muscle satellite cells would be compromised, and that nitric oxide (NO) supplementation would improve satellite cell activity in old muscle. Single intact myofibers were isolated from the gastrocnemius muscles of young (2mo), adult (10mo), and aged (22mo) mice. Fibers were centrifuged to stimulate...
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