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Binding of beta 2 glycoprotein I (β 2 GPI) to apoptotic cells plays a key role in the opsonization of apoptotic bodies and the formation of antiphospholipids antibodies. Here, we describe the binding of β 2 GPI to apoptotic cells using β 2 GPI labelled with biotin-hydrazide (β 2 GPI-bh) after oxidation of its glycan chains. Flow cytometry analyses and confocal microscopy...
Human apolipoprotein H (apo H) was found to bind specifically to hepatitis B surface antigen (HBsAg) from hepatitis B virus (HBV)-infected individuals. We used recombinant HBsAg proteins to analyze HBV domains recognized by apo H. We showed that the myristylated pre-S1 domain of HBsAg strongly interacted with apo H. This binding involved phospholipid components of the HBV envelope because their removal...
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