Search results for: P. Hinterdorfer

Items from 1 to 16 out of 16 results

article

Glycan distribution and density in native skin's stratum corneum

J. Danzberger, M. Donovan, C. Rankl, R. Zhu, more

Skin Research and Technology > 24 > 3 > 450 - 458

Background The glycosylation of proteins on the surface of corneocytes is believed to play an important role in cellular adhesion in the stratum corneum (SC) of human skin. Mapping with accuracy the localization of glycans on the surface of corneocytes through traditional methods of immunohistochemistry and electron microscopy remains a challenging task as both approaches lack enough resolution or...

chapter

Simultaneous Topography and Recognition Imaging

A. Ebner, L. A. Chtcheglova, J. Preiner, J. Tang, more

NanoScience and Technology > Scanning Probe Microscopy in Nanoscience and Nanotechnology > Characterization > 325-362

Summary Atomic force microscopy (AFM) has developed into a key technique for the investigation of biological samples. In contrast to other microscopy methods, high lateral resolution down to the nanometer scale and measurements under physiological conditions are possible. Additionally, the piconewton force sensitivity allows accurate data collection for single-molecule interactions. This chapter describes...

article

Single molecule binding dynamics measured with atomic force microscopy

M.H. van Es, J. Tang, J. Preiner, P. Hinterdorfer, more

Ultramicroscopy > 2014 > 140 > C > 32-36

We present a new method to analyse simultaneous Topography and RECognition Atomic Force Microscopy data such that it becomes possible to measure single molecule binding rates of surface bound proteins. We have validated this method on a model system comprising a S-layer surface modified with Strep-tagII for binding sites and strep-tactin bound to an Atomic Force Microscope tip through a flexible Poly-Ethylene-Glycol...

article

Simultaneous topography and recognition imaging on endothelial cells

L. A. Chtcheglova, P. Hinterdorfer

Journal of Molecular Recognition > 24 > 5 > 788 - 794

Determining the landscape of specific binding sites on biological samples with high spatial accuracy (in the order of several nanometres) is an important task in many fields of biological science. During the past five years, dynamic recognition imaging (e.g. simultaneous topography and recognition (TREC) imaging) has proven to be a powerful technique in biophysical research. This technique becomes...

article

AFM imaging of functionalized double-walled carbon nanotubes

C. Lamprecht, J. Danzberger, P. Lukanov, C.-M. Tîlmaciu, more

Ultramicroscopy > 2009 > 109 > 8 > 899-906

We present a comparative study of several non-covalent approaches to disperse, debundle and non-covalently functionalize double-walled carbon nanotubes (DWNTs). We investigated the ability of bovine serum albumin (BSA), phospholipids grafted onto amine-terminated polyethylene glycol (PL-PEG ₂₀₀₀ -NH ₂ ), as well as a combination thereof, to coat purified DWNTs. Topographical imaging...

article

Imaging and Detection of Single Molecule Recognition Events on Organic Semiconductor Surfaces

J. Preiner, N. S. Losilla, A. Ebner, P. Annibale, more

Nano Letters > 2009 > 9 > 2 > 571 - 575

article

Aldosterone receptor sites on plasma membrane of human vascular endothelium detected by a mechanical nanosensor

L. Wildling, P. Hinterdorfer, K. Kusche-Vihrog, Y. Treffner, more

Pflügers Archiv - European Journal of Physiology > 2009 > 458 > 2 > 223-230

The mineralocorticoid hormone aldosterone acts on target cells of kidney, colon, and the cardiovascular system through genomic and nongenomic pathways. Although the classical intracellular mineralocorticoid receptor plays a key role in mediating both pathways, it is unclear whether there are specific aldosterone receptors located on the cell surface. To search for such sites in vascular endothelium,...

article

Piezo-Aktoren und -Sensoren in der Rastersondenmikroskopie

B. Arminger, S. Adamsmair, F. Kienberger, P. Hinterdorfer, more

e & i Elektrotechnik und Informationstechnik > 2008 > 125 > 3 > 86-91

Piezoelektrische Materialien sind ein zentrales Element der Rastersondenmikroskopie. Dieser Artikel gibt einen Überblick über die dominanten Effekte, welche bei Piezos bei der Verwendung als Aktor bzw. Sensor auftreten. Dabei wird auf die Hysterese von Piezos eingangen und ein Verfahren vorgestellt, wie diese zum Teil kompensiert werden kann. Weiters wird das dynamische Verhalten von Piezoelementen...

article

Improving the contrast of topographical AFM images by a simple averaging filter

F. Kienberger, V.P. Pastushenko, G. Kada, T. Puntheeranurak, more

Ultramicroscopy > 2006 > 106 > 8-9 > 822-828

New image-processing methods were applied to atomic force microscopy images in order to visualize small details on the surface of virus particles and living cells. Polynomial line flattening and plane fitting of topographical images were performed as first step of the image processing. In a second step, a sliding window approach was used for low-pass filtering and data smoothing. The size of the filtering...

article

Single Molecule Recognition between Cytochrome C 551 and Gold-Immobilized Azurin by Force Spectroscopy

B. Bonanni, A.S.M. Kamruzzahan, A.R. Bizzarri, C. Rankl, more

Biophysical Journal > 2005 > 89 > 4 > 2783-2791

Recent developments in single molecule force spectroscopy have allowed investigating the interaction between two redox partners, Azurin and Cytochrome C 551. Azurin has been directly chemisorbed on a gold electrode whereas cytochrome c has been linked to the atomic force microscopy tip by means of a heterobifunctional flexible cross-linker. When recording force-distance cycles, molecular recognition...

article

Single molecule fluorescence and force microscopy

G.J. Schütz, P. Hinterdorfer

Experimental Gerontology > 2002 > 37 > 12 > 1495-1511

The investigation of biomolecules has entered a new age since the development of methodologies capable of studies at the level of single molecules. In biology, most molecules show a complex dynamical behavior, with individual motions and transitions between different states occurring highly correlated in space and time within an arrangement of various elements. Recent advances in the development of...

article

Highly ordered anisotropic nano-needles in para-sexiphenyl films

A. Andreev, H. Sitter, N.S. Sariciftci, C.J. Brabec, more

Thin Solid Films > 2002 > 403-404 > Complete > 444-448

Atomic force microscopy (AFM) was used to investigate the early growth stage of highly ordered para-sexiphenyl thin films deposited by hot wall epitaxy on mica, in order to find the process controlling parameters. It was shown that the growth time and surface type are important parameters for controlling of the film morphology, in terms of the degree of anisotropy and long range order. X-Ray diffraction...

article

Para- sexiphenyl/C ₆ ₀ bi- and multilayers grown by hot wall epitaxy

G. Matt, A. Andreev, H. Sitter, C.J. Brabec, more

Synthetic Metals > 2001 > 121 > 1-3 > 1373-1374

article

Self-assembled growth of highly oriented para- sexiphenyl thin films

A. Andreev, H. Sitter, C.J. Brabec, P. Hinterdorfer, more

Synthetic Metals > 2001 > 121 > 1-3 > 1379-1380

article

Recognition force microscopy/spectroscopy of ion channels: applications to the skeletal muscle Ca ² ⁺ release channel (RYR1)

G. Kada, L. Blayney, L.H. Jeyakumar, F. Kienberger, more

Ultramicroscopy > 2001 > 86 > 1-2 > 129-137

The skeletal muscle Ca ² ⁺ release channel (ryanodine receptor 1, RYR1) plays an important role in the excitation-contraction coupling process. We purified ryanodine receptor type 1 from rabbit white muscle and adsorbed it to mica sheets with the cytoplasmic side facing up. Single receptors of uniformly distributed size and shape of 10-12nm height and 40-50nm width, and occasionally some...

article

A NON-INVASIVE METHOD FOR THE TIGHT ANCHORING OF CELLS FOR SCANNING FORCE MICROSCOPY

K. SCHILCHER, P. HINTERDORFER, H.J. GRUBER, H. SCHINDLER

Cell Biology International > 1997 > 21 > 11 > 769-778

Use of scanning force microscopy (SFM) for high resolution imaging of cell surfaces requires the cells to be tightly attached to substrates. Imaging of loosely adhered RBL-2H3 cells enabled determination of the cell size and investigation of larger structures and pseudopodia but failed in resolving more detail. Immobilization under non-invasive conditions via flexible crosslinkers containing a hydrophobic...

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