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8-Nitroguanosine 3′,5′-cyclic monophosphate (8-nitro-cGMP) is formed endogenously under conditions of excess production of NO coupled with oxidative stress. Recently, we found that the electrophilic nitro moiety underwent nucleophilic substitution with hydrogen sulfide anion (HS − ) to yield the novel product 8-SH-cGMP. Endogenous 8-SH-cGMP levels are yet to be identified, however. In this...
The biological significance of nitrated guanine derivatives, especially 8-nitroguanosine 3′,5′-cyclic monophosphate (8-nitro-cGMP), has become evident. Therefore it is important to determine the presence and relative abundance of 8-nitro-cGMP formed in cells and tissues. In the present study, we performed immunocytochemistry with monoclonal antibodies specific for 8-nitroguanine (clone NO2-52) and...
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