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High temperature after anthesis may degrade the grain quality of rice (Oryza sativa L.). To understand the effect of stress duration (initial and mid grain filling stages) and the influence to grain quality at different spikelet positions, an indica cultivar and a japonica cultivar, Yangdao 6 and Yangjing 9538, were used in a pot experiment under 35 °C during the initial (8–18 d after anthesis) and...