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Time series data provided by single-molecule Förster resonance energy transfer (smFRET) experiments offer the opportunity to infer not only model parameters describing molecular complexes, e.g., rate constants, but also information about the model itself, e.g., the number of conformational states. Resolving whether such states exist or how many of them exist requires a careful approach to the problem...
By using single-molecule fluorescence resonance energy transfer (smFRET), we observe the real-time dynamic coupling between the ribosome, labeled at the L1 stalk, and transfer RNA (tRNA). We find that an interaction between the ribosomal L1 stalk and the newly deacylated tRNA is established spontaneously upon peptide bond formation; this event involves coupled movements of the L1 stalk and tRNAs as...
This paper reports a temperature-controlled microfluidic platform for single-molecule studies. Parallel microchannels are formed between glass and quartz, and are integrated with on-chip heaters and temperature sensors. Combined with surface derivatization and fluorescent labeling of biomolecules, temperature-dependent changes in the rates of fluorescence photobleaching are measured. The results demonstrate...
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