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We describe a terbium-ligand complex (TbL) for a microtiterplate assay for phosphate (P) in the 0.3–100μmolL −1 range based on luminescence quenching. As the pH optimum is at neutral pH (7.4) the probe is quenched by both, primary (H 2 PO 4− ) and secondary phosphate (HPO 42− ). The LOD is 110nmolL −1 . A Stern–Volmer study revealed that quenching...
We present a time-resolved (gated) luminescence-based method for determination of Cu 2+ ions in microtiterplate format in the nanomolar concentration range using the novel long-lived terbium-[1-methyl-4-hydroxy-3-(N-2-ethyl-5-aminothiadiazolyl-)-carbamoyl-quinoline-2-one] (TbL) complex. The probe works best in Tb:L=1:2 stoichiometry at neutral pH. The dynamic range is from 10 to 300nmolL ...
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