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Fragilysin, an extracellular zinc metalloprotease produced by enterotoxigenic strains of the anaerobic bacterium Bacteroides fragilis, disrupts the paracellular barrier by cleavage of the intercellular proteins between epithelial cells resulting in fluid secretion. Intranasal immunization of mice with fragilysin and co-administered ovalbumin (Ova) resulted in an Ova-specific serum IgG response that...
We have developed an episomal inducible gene expression system in Entamoeba histolytica based on the TetR repressor. The tetR gene was placed under control of 5′ and 3′ ferredoxin (fdx) regulatory sequences on a plasmid encoding the hygromycin resistance gene directed by 5′ and 3′ hgl sequences. The reporter luciferase constructs were introduced on a second episome bearing the neomycin resistance...
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