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In the straw biogas fermentation with high concentration, ratio of nitrogen and amount of seeding material have significant effect. Experiments show that gas production is very high in early time when the urea addition level is 2.5% or 3.0%, but the methane content of gas is less than 45%. when the urea proportion is 3.5%, the gas production curve is gently, the fermentation starting time is short...
Currently, the research on indoor air pollution had become one of the hot subjects in the field of environment science. Toluene diisocyanate was a kind of indoor air pollutant with low toxic, but the most toxic in all diisocyanate esters, which was recognized as a kind of industry allergen. It was studied in this issue that the effect of toluene diisocyanate ocontent on superoxide dismutase, malondialdehyde...
Toluene diisocyanate was a kind of indoor air pollutant with low toxic, but the most toxic in all diisocyanate esters, which was recognized as a kind of industry allergen. To study the effect on markers of oxidative damage and energy metabolism in blood induced by toluene diisocyanate(TDI). The mice contacted with TDI by respiratory tract in 50L static inhalation tank. The Exposure sustained two weeks,...
This paper is to determine metabolites of toluene diisocyanate (TDI) in mice and deduced the pathway for toluene diisocyanate metabolism in the organism. Toluene diisocyanate was administered to mice for 2 weeks, samples of blood, feces, and urine were taken, and the structure of the toluene diisocyanate metabolites was determined using gas chromatography-mass spectrometry (GC-MS). Conditions for...
To investigate the pro-apoptosis effect of isothiocyanates(ITCS) on human gastric adenoma cells SGC- 7901 and its mechanism. METHODS: SGC-7901 was treated with different concentrations of ITCS. MTT assay was used to evaluate the influence of ITCS on cell proliferation. Flow cytometry was used to test reactive oxygen species (ROS) levels, intracellular mitochondrial transmembrane potential (Deltapsim),...
This paper is to to determine metabolites of toluene diisocyanate (TDI) in mice and deduced the pathway for toluene diisocyanate metabolism in the organism. Toluene diisocyanate was administered to mice for 2 weeks, samples of blood, feces, and urine were taken, and the structure of the toluene diisocyanate metabolites was determined using gas chromatography-mass spectrometry (GC-MS). Conditions for...
This studies are carried on toxic action and machanism of toluene diisocyanate on immune system. With HE pigmentation and terminal deoxynucleotide transferase- mediated dUTP nick end labeling (TUNEL), effect on Eosinophilia numbers and eosinophil apoptosis of toluene diisocyanate in mice is observed. It is still observed that effect of toluene diisocyanate on conglutinate molecule and IL-4, IL-5,...
This paper is to study the effects of TDI on mice marrow cells . The effects of TDI were studied by laser scanning confocal microscope to investigate morphologic changing of the mice bone marrow cell and genotoxicity test -chromosomal aberration test and sister chromatic exchange (SCE) test. Results showed that the 1/16LC50 and 1/8LC50 dosage of TDI had no significant effects on the fluorescence pixels...
To observe the effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca24]; in the cells, and to uncover the mechanism by which solanine induces apoptosis. HepG2 cells are double stained with TMRE and Fluo- 3/AM, and both the change in membrane potential of mitochondria and that of [Ca2+]; in the cells are observed using LCSM.The results of double staining with TMRE and...
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