Search results for: Luc Michiels

Items from 1 to 8 out of 8 results

article

Unsupervised Machine Learning‐Based Clustering of Nanosized Fluorescent Extracellular Vesicles

Sören Kuypers, Nick Smisdom, Isabel Pintelon, Jean‐Pierre Timmermans, more

Small > 17 > 5 > n/a - n/a

Extracellular vesicles (EV) are biological nanoparticles that play an important role in cell‐to‐cell communication. The phenotypic profile of EV populations is a promising reporter of disease, with direct clinical diagnostic relevance. Yet, robust methods for quantifying the biomarker content of EV have been critically lacking, and require a single‐particle approach due to their inherent heterogeneous...

article

Direct detection of nano-scale extracellular vesicles derived from inflammation-triggered endothelial cells using surface plasmon resonance

Baharak Hosseinkhani, Nynke van den Akker, Jan D'Haen, Mick Gagliardi, more

Nanomedicine: Nanotechnology, Biology and Medicine > 2017 > 13 > 5 > 1663-1671

A major conceptual breakthrough in cell signaling has been the finding of EV as new biomarker shuttles in body fluids. Now, one of the major challenges in using these nanometer-sized biological entities as diagnostic marker is the development of translational methodologies to profile them. SPR offers a promising label-free and real time platform with a high potential for biomarker detection. Therefore,...

article

Polarization second harmonic generation by image correlation spectroscopy on collagen type I hydrogels

Rik Paesen, Kathleen Sanen, Nick Smisdom, Luc Michiels, more

Acta Biomaterialia > 2014 > 10 > 5 > 2036-2042

Successful engineering of biomimetic tissue relies on an accurate quantification of the mechanical properties of the selected scaffold. To improve this quantification, typical bulk rheological measurements are often complemented with microscopic techniques, including label-free second harmonic generation (SHG) imaging. Image correlation spectroscopy (ICS) has been applied to obtain quantitative information...

article

Construction of helper plasmid-mediated dual-display phage for autoantibody screening in serum

Kaushik Rajaram, Veronique Vermeeren, Klaartje Somers, Veerle Somers, more

Applied Microbiology and Biotechnology > 2014 > 98 > 14 > 6365-6373

M13 filamentous bacteriophage has been used in displaying disease-specific antibodies, biomarkers, and peptides. One of the major drawbacks of using phage in diagnostic assays is the aspecific adsorption of proteins leading to a high background signal and decreasing sensitivity. To deal with this, we developed a genetically pure, exchangeable dual-display phage system in which biomarkers and streptavidin-binding...

article

Nanocrystalline diamond impedimetric aptasensor for the label-free detection of human IgE

Dinh T. Tran, Veronique Vermeeren, Lars Grieten, Sylvia Wenmackers, more

Biosensors and Bioelectronics > 2011 > 26 > 6 > 2987-2993

Like antibodies, aptamers are highly valuable as bioreceptor molecules for protein biomarkers because of their excellent selectivity, specificity and stability. The integration of aptamers with semiconducting materials offers great potential for the development of reliable aptasensors. In this paper we present an aptamer-based impedimetric biosensor using a nanocrystalline diamond (NCD) film as a...

article

Structural and Optical Properties of DNA Layers Covalently Attached to Diamond Surfaces

Sylvia Wenmackers, Simona D. Pop, Katy Roodenko, Veronique Vermeeren, more

Langmuir > 2008 > 24 > 14 > 7269 - 7277

article

Human chorionic gonadotropin (hCG) and prevention of breast cancer

Jaak Ph. Janssens, José Russo, Irma Russo, Luc Michiels, more

Molecular and Cellular Endocrinology > 2007 > 269 > 1-2 > 93-98

Animal and ‘in vitro’ experiences learned that human chorionic gonadotropin (hCG) is capable to protect from breast cancer. Receptors for hCG/luteinizing hormone (LH) are present on human female and male breast cancer cells. hCG decreases proliferation and invasion of breast cancer MCF-7 cells by inhibiting NF-kappa B, AP-1 activation and other genes. Doxorubicin toxicity is enhanced by conjugation...

article

A European Multicenter Study of Phenylalanine Hydroxylase Deficiency: Classification of 105 Mutations and a General System for Genotype-Based Prediction of Metabolic Phenotype

Per Guldberg, Françoise Rey, Johannes Zschocke, Valentino Romano, more

The American Journal of Human Genetics > 1998 > 63 > 1 > 71-79

Phenylketonuria (PKU) and mild hyperphenylalaninemia (MHP) are allelic disorders caused by mutations in the gene encoding phenylalanine hydroxylase (PAH). Previous studies have suggested that the highly variable metabolic phenotypes of PAH deficiency correlate with PAH genotypes. We identified both causative mutations in 686 patients from seven European centers. On the basis of the phenotypic characteristics...

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