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Several studies indicated that biopharmaceuticals based on the recombinant protein E7 of human papillomavirus (HPV) can serve as therapeutic vaccines preventing the development of cancer in women infected with high-risk types of HPV such as HPV16. Here, we report effective extraction and purification of a plant-produced E7GGG-lichenase fusion protein, an HPV16 subunit vaccine candidate, from Nicotiana...
Alfalfa mosaic virus coat protein or its messenger RNA is required in the inoculum for virus infection. The N-terminus of the coat protein is required for activity; thus, changes were made in the amino acid sequence of this region. Six coat protein mutants were tested for activity in virus infection assays in protoplasts. A coat protein mutant in which N-terminal residues 3-19 were absent was inactive;...
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