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Bath application of 5-HT (1-1000 μM) induced a tetrodotoxin (TTX)-resistant outward current at the holding membrane potential (VH) of −50 mV in 104/162 (64.2%) of substantia gelatinosa (SG) neurons from the rat spinal cord in vitro. The 5-HT-induced outward current was suppressed by an external solution containing Ba2+, or a pipette solution containing Cs2SO4 and tetraethylammonium. It was reversed...