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The product of the human ob (obesity) gene, leptin, appears to function in the maintenance of body weight in vivo. When injected into mice, this hormone reduces food consumption and causes weight loss. This work has been done with recombinant leptin (re-leptin) purified and renatured from inclusion bodies in Escherichia coli. We have expressed the human obesity gene encoding the predicted full-length...
Porcine epithelial cells were cultivated on microspheres with an average diameter of 35 [mu ]m in a stirred tank bioreactor for virus production. The oxygen uptake rate (OUR) was determined on-line by a dynamic method. This on-line measurement was used to estimate glucose consumption rate for perfusion rate adjustment. Using this on-line technique, the time points for virus infection and harvest...
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