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Simple methods for detection and isolation of protein-porphyrin complexes were elaborated in our laboratory. They are based on the separation of protein-porphyrin complexes in native polyacrylamide gel and measurement of their fluorescence, with the use of two detection systems: the commercially available Gel Doc™ 2000 system, and a system specially designed for the purpose of these investigations,...
The article contains information regarding the similarities and differences between proteins of the thiol-activated cytolysins family (TACY). Members of this group of haemolysins are produced by several species of Gram-positive bacteria (Listeria spp., Streptococcus spp., Bacillus spp., Clostridium spp.,) and also by Gram-negative Klebsiella spp.. Their cytolytic activity is sensitive to oxygen and...
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