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Opracowano metodę otrzymywania haploidów owsa metodą izolowanych pylników. Materiał roślinny stanowiło 25 genotypów owsa. Rośliny donorowe rosły w warunkach szklarniowych, a kłosy ścinano, gdy większość mikrospor znajdowała się w fazie późnojednojądrowej. Pylniki wykładano na pożywki różniące się składem mikro-, makroelementów, witamin oraz stężeniami regulatorów wzrostu. Po 6-8 tygodniach kultury...
Seedlings of winter rape were cultured in vitro on media containing 24-epibrassinolide, EBR (100 nM) and cadmium (300 µM). After 14 d of growth, fast fluorescence kinetics of chlorophyll (Chl) a and contents of photosynthetic pigments and Cd in cotyledons were measured. Cd was strongly accumulated but its content in cotyledons was 14.7 % smaller in the presence of EBR. Neither Cd nor EBR influenced...
The suspension culture was initiated from callus derived from immature inflorescences of winter wheat var Almari. The aim of our investigation was evaluation of the influence of twice increased sucrose concentration in the medium on the physicochemical properties and cell morphology during 15 days of suspension culture. The number of cells, volume, weight, shape and viability were monitored in a few...
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